Research Paper Volume 12, Issue 18 pp 18238—18250
The mitochondrial-targeted peptide SBT-20 ameliorates inflammation and oxidative stress in chronic renal failure
Figure 1. The location and effect of SBT-20 in mitochondrial. (A) MTT assay was carried out to assess the cell viability. n=10. (B) Representative diagram of cellular uptake of SBT-20. (C) Representative diagram of colocalization of SBT-20 with FITC. (D) Representative diagram of colocalization of SBT-20 with Mfn2. (E) The mRNA level of inflammatory-associated cytokine IL-1β, IL-6, NF-κB1 and NF-κB2 in different group. n=7. (F) The protein level of NF-κB-p65 and TNFα. (G) The ROS production was measured in cells. n=5. (H) Detection of mitochondrial membrane potential. (*P<0.05 vs. control group, #P<0.05 vs. LPS group).