Research Paper Volume 12, Issue 18 pp 18238—18250
The mitochondrial-targeted peptide SBT-20 ameliorates inflammation and oxidative stress in chronic renal failure
Figure 2. SBT-20 blocks oxidative stress induced by H2O2 in HK-2 cells. (A) MTT assay was carried out to assess the cell viability. n=10. (B) RT-PCR was employed for detecting oxidative stress-relative markers, HIF-1α, HO-1, SOD1 and SOD2. n=6. (C) The ROS production was measured in cells. (D) Detection of mitochondrial membrane potential. (E) The apoptotic cells was quantified using flow cytometry. n=3. (F) The protein level of cleaved-caspase3 was assessed. n=5. (*P<0.05 vs. control group, #P<0.05 vs. H2O2 group).