Research Paper Volume 12, Issue 17 pp 17271—17287
Profiling of circular RNAs in age-related cataract reveals circZNF292 as an antioxidant by sponging miR-23b-3p
Figure 5. Verification and the interaction network of selected circRNAs in ARC. (A) qRT-PCR validation of candidate circRNAs in the control and ARC groups (*, P < 0.05). (B) The log2FC (fold change) of candidate circRNAs with significant difference by RNA-Seq and qRT-PCR. (C) Validation of miR-23b-3p in the normal and ARC groups. (D) A circRNA-miRNA-mRNA interaction map. The purplish red arrowheads represent circRNAs, the green ovals represent cataract-related miRNAs, and the blue hexagons represent the target genes related to oxidative stress. StarBase and TargetScan were used to predict the target mRNAs of these miRNAs, among which the oxidative stress related genes of miR-23b-3p are GPX3, SIRT1, PRDX6, ALDH6A1, and SOD1.