Research Paper Volume 12, Issue 16 pp 16368—16389

Figure 5. FRT played a role in radiation protection by regulating PARP-1/AIF. KO mice and WT mice were pretreated with FRT, then after 4 days irradiated with a dose of 6 Gy. Thymus tissue was removed 4 days after radiation. (A) RNA was extracted using a homogenizer and the expression of PARP-1 gene was quantified using fluorescent quantitative PCR. PARP-1 gene expression was increased after irradiation of WT mice. FRT was effective in inhibiting activation of PARP-1 after irradiation (*** P<0.01 compared with the control group; ### P<0.01 compared with radiation group). Data was expressed as mean ±SD, n=5. (B) AIF gene expression increased after irradiation in WT mice. FRT was effective in inhibiting activation of AIF after irradiation (*** P<0.01 compared with the control group; ### P<0.01 compared with radiation group). Data was expressed as mean ±SD, n=5. (C) In the thymus tissues of KO mice, there was no significant difference in AIF gene expressions between the control, radiation group and FRT drug groups (NS, not significant). Data was expressed as mean ±SD, n=5. (D) Proteins in thymus tissues were extracted by RIPA lysis buffer. Protein expressions of PARP-1 and AIF, as measured by Western blot analysis, increased after irradiation in WT mice. FRT effectively inhibited the activation of PARP-1 and AIF after irradiation. In the thymus tissues of KO mice, there was no significant difference in AIF protein expressions between the control, radiation group and FRT drug groups (NS, not significant; *** P<0.01 compared with the control group; ### P<0.01 compared with radiation group). Data was expressed as mean ±SD, n=5.