Figure 1. p38γ expression is elevated in human OS tissues and OS cells. Expression of p38γ mRNA (A, C) and protein (B, D) in twelve (n=12, derived from twelve different OS patients) primary human osteosarcoma tissues (“T”) and matched surrounding normal bone tissues (“N”), as well as in U2OS established OS cells, primary human OS cells (OS1/OS2/OS3, derived from three different OS patients), OB-6 osteoblastic cells, and primary human osteoblasts are shown. Expression of listed proteins was quantified and normalized to the loading control (B, D). Data presented as mean ± standard deviation (SD). *** p< 0.001 vs. “N” tissues/osteoblasts.