Research Paper Volume 12, Issue 16 pp 16437—16456

Figure 5. KCNN4 was upregulated in PTC cell lines and promoted their proliferation in vitro. (A) The relative expression of KCNN4 in PTC cell lines (compared with GAPDH). KCNN4 was upregulated in three PTC cell lines (KTC-1, TPC-1 and BCPAP) compared to the normal thyroid cell line HTORI-3. (B) KCNN4 expression was lower in the Si-KCNN4 group than in the Si-NC group in the three PTC cell lines. (C) KCNN4 protein expression was lower in the Si-KCNN4 group than in the Si-NC group. (D, E) CCK-8 assays and colony formation assays in the three PTC cell lines. Two-way analysis of variance was used to analyze the CCK-8 assay data, and Student’s t-test was used for the others. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.