Research Paper Volume 12, Issue 17 pp 17436—17458

Figure 2. Ultrastructure and morphometry of sciatic nerves under transmission electron microscopy. Representative images of (A) cross-sections and (B) longitudinal sections of sciatic nerves from different groups at magnifications of 8000×, 20000×, and 80000×; scale bars, 2 μm, 500 nm, and 200 nm, respectively. Morphometric analyses of sciatic nerve showing (C) the number of myelinated axons per 5,000 μm² and (D) the percentage of abnormal myelin fibers in different groups. Means ± SD; n = 3/group. (E) Quantification of g ratios for axons in different groups. n=632 axons from 3 normal control rats, n=525 axons from 3 distilled water-treated DPN rats, n=585 axons from JMT-treated DPN rats, n=544 axons from 3 NTP-treated DPN rats. ***p < 0.001, *p < 0.05, vs. normal control group; †††p < 0.001, †p < 0.05 vs. distilled water-treated DPN group; ‡‡‡p < 0.001 vs. JMT-treated DPN group. One-way ANOVA followed by Tukey’s multiple comparison test or Kruskal-Wallis test followed by Dunn’s multiple comparisons test. Pound signs (#) indicate onion-bulb and bubble form protrusions. Red arrows indicate lamellar separation between the axon and myelin sheath and demyelination.