Research Paper Volume 12, Issue 15 pp 15546—15555
Glycochenodeoxycholic acid induces stemness and chemoresistance via the STAT3 signaling pathway in hepatocellular carcinoma cells
Figure 3. GCDC promotes EMT and stemness in HCC cells. (A, B) Reverse-transcriptase polymerase chain reaction (RT-PCR) was used to detect the expression of stem cell markers (Sox2, Sox9, Nanog, and CD133) in HCC cells. *P<0.05, **P<0.01, ***P<0.001. (C) Western blotting was used to detect the expression of stem cell markers (Sox2, Sox9, Nanog, and CD133) in HCC cells. (D, E) RT-PCR was performed to examine the expression of E-cadherin and vimentin in HCC cells. *P<0.05, **P<0.01. (F, G) The protein expression of E-cadherin and vimentin was confirmed by immunofluorescence.