Research Paper Volume 12, Issue 17 pp 17459—17479

Figure 6. ABCB1 facilitates drug efflux of RCC cells. (A) Cell cycles were detected in resistant cells after LINC00160 knockdown and overexpression. (B) Western blotting analysis of ABCB1 after silencing LINC00106 and SAA1 in resistant cells respectively, upregulating LINC00160 and SAA1 in parental cells respectively. β-actin served as the loading control. (C) Resistant cell lysate was subjected to immunoprecipitation with anti-ABCB1 antibody and analyzed by western blotting. Each experiment was performed at least three times and data was represented as mean ± SEM. P>0.05 is denoted by ns. ABCB1, ATP binding cassette subfamily B member 1; RCC, renal cell carcinoma; LINC00160, long non-coding RNA 160; SAA1, serum amyloid A1.