Research Paper Volume 12, Issue 17 pp 17601—17624

Figure 5. A subset of newly generated podocytes (tdTomato⁺EGFP⁺) from PEC origin co-express podocin in the glomerular tuft of aged mice. (A–D) Representative confocal images of tdTomato⁺ (red), EGFP⁺ (green) and podocin staining (blue) in young and aged mice. The inserts show separate channels of the outlined glomeruli, with superscripts: 1=tdTomato, 2=EGFP, 3=podocin and 4=merged. Podocin staining was detected with an antibody, tdTomato and EGFP reporters were detected without antibody. (A) Young mice (OC) showed that tdTomato⁺PECs (red) are detected along Bowman’s capsule (A¹). The majority of EGFP⁺ cells (green) (A²) co-localize with podocin (blue) (A³) and create a cyan color (A⁴). (B) Aged mice (OC) showed that a subset of differentiated tdTomato⁺ PECs (red) (B¹) co-expresses EGFP⁺ (green) (B²) and podocin (blue) (B³) creating a pink/white color in the glomerular tuft (white arrows) (B⁴). (C) Young mice (JMC) showed that tdTomato⁺PECs (red) (C¹) are detected along Bowman’s capsule. The majority of EGFP⁺ cells (green) (C²) co-localize with podocin (blue) (C³) in the glomerular tuft creating a cyan color (C⁴). (D) Aged mice (JMC) showed that a higher number of tdTomato⁺PECs (red) (D¹) have migrated to the glomerular tuft (marked with white arrows), become EGFP⁺ (D²) and co-localized with podocin (blue) (D³), creating a pink/white color (D⁴). Scale bars represent 25μm or 5μm (insets).