Research Paper Volume 12, Issue 15 pp 15756—15770
MiR-483 induces senescence of human adipose-derived mesenchymal stem cells through IGF1 inhibition
Figure 1. Characterization and verification of senescent MSCs from human adipose tissues from two different donors (#1 and #2). (A) Oil Red O staining of hADSCs at P9 and P16 (10×; scale: 50 μm); MRNA expression of PPARγ was evaluated by RT-qPCR. (B) Alkaline phosphatase staining of hADSCs at P9 and P16 (10×; scale: 50 μm); MRNA expression of RUNX2 was evaluated by RT-qPCR. (C) MRNA expression levels of p16, p21, and p53 were evaluated by RT-qPCR; western blot analysis of p53. (D) SA-β-gal staining of hADSCs at P9 and P16 (10×; scale: 50 μm). (E) The proliferation of hADSCs at P9 and P16 was detected by CCK-8 assay. (F) Telomere length was demonstrated by qPCR.