Research Paper Volume 12, Issue 17 pp 17634—17646
DUSP4 directly deubiquitinates and stabilizes Smad4 protein, promoting proliferation and metastasis of colorectal cancer cells
Figure 4. DUSP4 down-regulated Smad4 gene expression. (A) Western blot analysis of Smad4 protein expression in DUSP4 knockdown-treated SW480 cells and DUSP4 overexpression-treated HCT116 cells. (B) qRT-PCR analysis of Smad4 mRNA expression in DUSP4 knockdown-treated SW480 cells and DUSP4 overexpression-treated HCT116 cells. (C) Correlation analysis of DUSP4 protein and Smad4 protein in clinical samples. (D) Correlation analysis of DUSP4 mRNA and Smad4 mRNA in clinical samples. (E) Relationship analysis of DUSP4 and Smad4 in HEK293T cells which was transfected with DUSP4 and Smad4. (F–H) Relative Smad4 protein expressions in DUSP4 knockdown-treated SW480 cells and DUSP4 overexpression-treated HCT116 cells with cyclohexane (CHX) treatment at different time points. **P<0.01, ***P<0.001.