Research Paper Volume 12, Issue 20 pp 20396—20412

Figure 5. FAM83C-AS1 mediated CRC progression by stabilizing EZH2 protein to decrease SEMA3F expression. (A) The efficiency of EZH2 overexpression or SEMA3F knockdown was estimated using RT-qPCR. (B) SEMA3F expression in transfected cells was examined using RT-qPCR and western blot assay. (C, D) The proliferation ability of transfected cells was analyzed using CCK-8 and colony formation assays. (E, F) Wound healing and transwell assays were used to evaluate the migrating and invading abilities of tumor cells in different groups. (G) EMT process in transfected cells was measured using western blotting analysis and immunofluorescence assay. (H) The sphere formation ability of transfected cells was assessed using sphere formation assay. (I) Cell stemness in different groups was analyzed using flow cytometer analysis. ^**P < 0.01.