Research Paper Volume 12, Issue 20 pp 20396—20412

Figure 6. FAM83C-AS1 promotes tumorigenesis of CRC in vivo by inhibiting SEMA3F expression. (A–C) Transfected RKO cells were subcutaneously injected into nude mice. Tumor growth curve was obtained by recording tumor volume every 4 days. After 28 days, mice were sacrificed for further evaluation of tumor volume and weight. (D) Expression of FAM83C-AS1, EZH2 and SEMA3F in different groups was detected using RT-qPCR. (E) Protein expression of EZH2 and SEMA3F in different groups was detected via western blotting analysis. (F) Expression of Ki67, PCNA, E-cadherin and N-cadherin in different groups was analyzed using immunohistochemistry assay. (G) HE staining for metastasis nodes in lungs was used for evaluating cell metastasis in different groups. ^**P < 0.01. n.s.: no significance.