Research Paper Volume 12, Issue 16 pp 16579—16596

Figure 7. Inhibiting ER stress suppresses light-related autophagy. 661W cells/ARPE-19 cells were treated with SAL (20 μM for 661W cells; 10 μM for ARPE-19 cells) or vehicle and cultured under light/dark conditions for 3 days. The level of BECN1 and LC3BII in the whole cell lysate were determined with western blotting, and β-actin was referenced as an internal control. Three independent experiments are conducted two weeks apart. The results are presented as the mean± SEM. n (per group) =3, NS: no significance, *P < 0.05, **P < 0.01.