Research Paper Volume 12, Issue 18 pp 18571—18587

Figure 4. SNHG22 regulated endothelial cell function. (A) Representative images of HUVEC wound healing assays in the presence of U2OS supernatant. Scale bar: 100 μm. (B) Relative cell migration rates of HUVECs were measured. n=5; ^*P<0.05 by t-test. (C) Cell viability of HUVECs was assessed by CCK-8 assay. n=5; ^*P<0.05, ^**P<0.01. (D) Representative images of invading HUVECs. U2OS supernatant was included in all cultures. Scale bar: 200 μm. (E) The number of invading HUVECs were counted and are shown in the violin plots. n=5; ^**P<0.01 by t-test. (F) The level of VEGF in U2OS supernatant. Results are shown in the violin plots. n=5; ^***P<0.001 by t-test. (G) Representative images of HUVEC wound healing assays in the presence of HOS supernatant. Scale bar: 100 μm. (H) Relative cell migration rates of HUVECs were measured. n=5; ^*P<0.05, ^**P<0.01 by t-test. (I) Cell viability of HUVECs was assessed by CCK-8 assays. n=5; ^*P<0.05, ^**P<0.01. (J) Representative images of invading HUVECs. HOS supernatant was included in all cultures. Scale bar: 200 μm. (K) The number of invading HUVECs were counted and are shown in the violin plots. n=5; ^**P<0.01, ^***P<0.001 by the t-test. (L) The level of VEGF in HOS supernatant. Results are shown in the violin plots. n=5; ^***P<0.001 by the t-test.