Research Paper Volume 12, Issue 16 pp 15995—16020

Figure 5. Proteasome functionality in brain homogenates of 3xTg-AD mice. The ChT-L, T-L, PGPH and BrAAP components of the 20S proteasome and the ChT-L activity of the 26S proteasome were measured using fluorogenic peptides as reported in the Material and Method section (panel A) (^*p<0.05, ^**p<0.01 and ^***p<0.001 vs T0, ^# p<0.05, ^## p<0.01 and ^###p<0.001 vs C, ^§p<0.05, ^§§p<0.01 and ^§§§p<0.001 vs LAB). Immunodetection of the proteasome substrates p27, p53 and Ub-protein conjugates in brain homogenates of 3xTg-AD mice, T0, C, LAB and p62-LAB groups (panel B) and related densitometry (panel C). GAPDH was used as a control to check equal protein loading (^*p<0.05, ^**p<0.01 and ^***p<0.001 vs T0, ^#p<0.05, ^## p<0.01 and ^###p<0.001 vs C, ^§p<0.05 and ^§§p<0.01 vs LAB).