Expression of exosomal UCA1 in patients with esophageal cancer. (A) Expression of exosomal UCA1 from the plasma of patients with esophageal cancer (Tumor) or control subjects (Control). (B) ROC curves analysis of exosomal UCA1. (C) Expression of exosomal UCA1 in the plasma samples placed at room temperature for 0 h, 4 h, 8 h, and 24 h, respectively. (D) Expression of exosomal UCA1 in the plasma samples after 0, 2, 3, 4 freezing-thawing cycles. (*) denotes the difference between groups (P<0.05). Each experiment had a minimum of three replicates per treatment group.