Research Paper Volume 12, Issue 20 pp 20523—20539

Effect of exosomal UCA1 on tumor growth in vivo. (A) Representative images of nude mice treated with EC18 cells transfected with UCA1-expressing vector (UCA1-vector) or negative control vector (Control), or exosomes derived from NEEC cells transfected with UCA1-expressing plasmids (UCA1-Exo) or negative control plasmids (Control- Exo). (B) Tumor volumes of mice treated with EC18 cells transfected with UCA1-expressing vector (UCA1-vector) or negative control vector (Control), or exosomes derived from NEEC cells transfected with UCA1-expressing plasmids (UCA1-Exo) or negative control plasmids (Control- Exo). (C, D) Representative images of tumor tissues and tumor weights of mice treated with EC18 cells transfected with UCA1-expressing vector (UCA1-vector) or negative control vector (Control), or exosomes derived from NEEC cells transfected with UCA1-expressing plasmids (UCA1-Exo) or negative control plasmids (Control- Exo). (E) H&E staining assay for Ki67 expression in tumor tissues. (F) Immunohistochemistry assay for Ki67 expression in tumor tissues. (G) Putative binding site of miRNA-613 in UCA1. (H) Luciferase reporter assay. (I) Expression of miRNA-613 in esophageal cancer tissues (Tumor) and paired normal tissues (Control). (J) Pearson’s correlation between expressions of miRNA-613 and UCA1 in esophageal cancer tissues. (*) denotes the difference between groups (P<0.05). Each experiment had a minimum of three replicates per treatment group.