Research Paper Volume 12, Issue 20 pp 20702—20727

BAG5 is upregulated in DNA damage- and oxidative stress-treated cells but not in hypoxia-treated cells. (A–C) U2OS, HeLa, and SH-SY5Y cells were treated with 10 μM of etoposide for 48 h, 250 μM of H₂O₂ for 24 h, 10 μM of DFX for 48 h, or solvent. RNA was extracted, and BAG5 transcripts were detected by RT-Q-PCR. The relative fold in BAG5 mRNA expression was normalized to the GAPDH control and the solvent control (upper panel). The protein expression of BAG5, p53, HIF-1α, and β-actin was detected by Western blotting (lower panel). The relative fold in protein expression was normalized to that of the internal control β-actin and standardized with the solvent control. Error bars represent the SD of the means calculated using data from three independent experiments (Student’s t-test; *, p < 0.05, **, p < 0.01, ***, p < 0.001).