Research Paper Volume 12, Issue 20 pp 20753—20777
Substance P blocks ovariectomy-induced bone loss by modulating inflammation and potentiating stem cell function
SP facilitates the development of Treg under osteoporosis condition. The ratios of CD4+IL17+ cells in splenocytes (A) and in MLN cells (B) were analyzed by FACS analysis. The ratios of CD4+Foxp3+ cells in splenocytes (C) and in MLN cells (D) were quantified by FACS analysis. (E–G) The concentrations of TNF-α, IL-10 and IL-17 in serum were analyzed by ELISA. p values of less than 0.05 were considered statistically significant (* p <0.05, ** p <0.01, *** p <0.001). MLN: mesenteric lymph node. n = 12/group.