Research Paper Volume 13, Issue 3 pp 3428—3442
Reduced SULT2B1b expression alleviates ox-LDL-induced inflammation by upregulating miR-148-3P via inhibiting the IKKβ/NF-κB pathway in macrophages
Figure 4. SULT2B1b regulates the IKKβ/IκB/NF-κB signalling pathway through miR-148a-3p. (A, B) The expression levels of IKKβ, IκB and phosphorylated nuclear factor-kappa B (p-p65) protein were determined by Western blotting in Raw264.7 cells transfected with Ad-GFP or Ad-shSULT2B1b. (C) Raw264.7 cells were transfected with Ad-GFP or Ad-shSULT2B1b and stimulated with ox-LDL. Then, the cells were transfected with miR-148a-3p inhibitor (C, D) or miR-148a-3p mimic (E, F). IKKβ, IκB and p-p65 expression levels were determined by Western blotting (C, E). β-actin was used as a reference control. The relative levels of IKKβ, IκB and phosphorylated p65 were quantitively compared (D, F). Data are shown as the mean±SD of at least three independent experiments. **p<0.01, ***p<0.001. ns, no significant difference.