Research Paper Volume 13, Issue 3 pp 3588—3604

Figure 5. Overexpression of miR-106a reverses the effects of circFASTKD1. (A, B) CCK-8 assays were used to determine the growth curves of HUVECs (A) or HCMECs (B) transfected with the indicated vectors. (C) Photographs from the tube formation, cell migration and wound healing assays in HUVECs. (D–F) Quantitative analyses of the tube formation (D), cell migration (E) and wound healing (F) assays in HUVECs. (G) Photographs from the tube formation, cell migration and wound healing assays in HCMECs. (H–J) Quantitative analyses of the tube formation (H), cell migration (I) and wound healing (J) assays in HCMECs. (K, M) Western blotting analyses of LATS1, LATS2, YAP and p-YAP protein levels in HUVECs (K) and HCMECs (M) following the rescue experiments. (L, N) Western blotting analyses of the subcellular levels of YAP and p-YAP in HUVECs (L) and HCMECs (N). Data are presented as the mean of three experiments, and the error bars represent the SD (*P<0.05 and **P<0.01).