Figure 6. The results of AKR1C3 and AKR1D1 lentiviral transfection. (A) Relative protein expression of AKR1C3 and AKR1D1 in HCC cell lines. (B) The protein level of AKR1C3 in SMMC-7721 cells after the knockdown. (C) The cell viability of sh-AKR1C3 and control groups. (D) The protein level of AKR1D1 in HuH-7 cells after overexpression. (E) The cell viability of AKR1D1-1 and control groups. (F, G) The protein levels of MEK1, p-MEK1, p-Erk1/2, AR, and ID1 in the AKR1C3 knockdown and AKR1D1 overexpression cells.