Research Paper Volume 13, Issue 3 pp 4335—4356

Figure 3. MSCs attenuate cerebral I/R-induced injury in co-cultured neurons. (A) Viability of co-cultured neurons as assessed by MTT assay. (B) Apoptotic cell death in co-cultured neurons as detected by LDH-leakage assay. (C, D) Apoptotic cell death in co-cultured neurons as detected by Hoechst staining. (E) ROS production in co-cultured neurons as detected by DCFH-DA assay. (F) MDA production in co-cultured neurons as evaluated by lipid peroxidation MDA assay. (G) SOD production in co-cultured neurons as determined by WST-8 assay. (H) T-AOC level in co-cultured neurons as detected by ABTS assay. (I, J) CUEDC2 protein expression in co-cultured neurons as assayed by western blotting. (K, L) CUEDC2 protein expression in MSCs-treated brain tissues as assayed by western blotting. CTR, control; CUEDC2: CUE domain-containing 2; OGD/R: oxygen-glucose deprivation (4 hours) and reperfusion (24 hours); MSCs: mesenchymal stem cells; MCAO, middle cerebral artery occlusion. All data are presented as the mean value ± SD. *p<0.05, **p<0.01; compared to OGD/R + MSCs group, MCAO + MSCs treatment group.