Research Paper Volume 13, Issue 3 pp 4409—4427

Figure 2. MD2 deficiency alleviated Ang II induced vascular remodeling in vivo. (A) Aortic thickening in the mice and aortic tunica media fibrosis was examined by Masson trichrome staining and H&E staining (scale bar = 50 μm). (B) Quantification for staining results in 2A (n = 10; ***p<0.001 compared to Vehicle; ###p<0.001 compared to Ang II). (C) Representative immunofluorescence staining images for α-SMA (green) and Vimentin (red) in aortas. Tissues were counterstained with DAPI (blue) (scale bar = 50 μm). (D, E) Quantification for staining results in 2C (n = 10; ***p<0.001 compared to Vehicle; ##p<0.01 compared to Ang II). (F) Representative images of COL-3 and PCNA staining of aortas (scale bar = 500 μm; DAB chromogen staining (brown). (G, H) Quantification for staining results in 2F (n = 10; ***p<0.001 compared to Vehicle; ##p<0.01 compared to Ang II). (I, J) Expressions of α-SMA, Vimentin and COL-3 in the whole aorta (n = 10; *p<0.05, **p<0.01 compared to Vehicle; ##p<0.01 compared to Ang II).