Research Paper Volume 13, Issue 3 pp 4409—4427

Figure 3. MD2 deficiency alleviated Ang II induced inflammation and oxidative stress in vivo. (A) TNF-α and IL-6 mRNA levels in the aortas were detected using real-time qPCR assay (n = 10; ***p<0.001 compared to Vehicle; ##p<0.01 compared to Ang II). (B, C) Representative images of F4/80 and TNF-α staining of aortas (scale bar = 50 μm; DAB chromogen staining (brown)). (D) Quantification for staining results in 3B-C ((n = 10; **p<0.01, ***p<0.001 compared to Vehicle; ###p<0.001 compared to Ang II). (E) Levels of superoxide dismutase (SOD) and malondialdehyde (MDA) in the aortas (n = 10; **p<0.01, ***p<0.001 compared to Vehicle; #p<0.05, ##p<0.01 compared to Ang II). (F) Oxidative damage in the aortas as assessed by immunoreactivity to 3-nitrotyrosine (3-NT). Detection was performed by DAB (brown) (scale bar = 50 μm). (G) Representative images of dihydroethidium (DHE) staining in the aortas (scale bar = 50 μm). (H) Quantification for staining results in 3F-G (n = 10; ***p<0.001 compared to Vehicle; ##p<0.01 compared to Ang II).