Research Paper Volume 13, Issue 3 pp 4409—4427

Figure 6. MD2 knockdown alleviated Ang II-induced ROS by activation of SIRT-1. (A) VSMCs were transfected with siRNA against MD2 for 6 h and then incubated with Ang II for 4 h (panel A–E) and 24 h (panel F–G). (A) Levels of superoxide dismutase (SOD) and malondialdehyde (MDA) in VSMCs (n = 3; **p<0.01 compared to Ctrl; #p<0.05, ##p<0.01 compared to Ang II). (C–E) Superoxide production was measured by DCFH-DA (green) and DHE (red) (scale bar = 50 μm) (n = 3; ***p<0.001 compared to Ctrl; ##p<0.01 compared to Ang II). (F, G) Expressions of SIRT-1and NOX4 in the cultural medium were detected by western blot (n = 3; ***p<0.001 compared to Ctrl; ##p<0.01, ###p<0.001 compared to Ang II). (H) Representative immunofluorescence staining images for SIRT-1 (red) in mouse aortas (scale bar = 50 μm). (I) Quantification for staining results in panel I (n = 10; ***p<0.001 compared to Vehicle; ###p<0.001 compared to Ang II).