Research Paper Volume 13, Issue 3 pp 4468—4481

Figure 1. PRMT5 is highly expressed in human colorectal cancer cells and tissues. (A) The mRNA expression level of PRMT5 in various human colorectal tumor cell lines and normal colonic mucosal FHC cells (n=4). **P < 0.01 vs. FHC cells. (B) Western blot analysis of PRMT5 protein expression level in various human colorectal tumor cell lines and normal colonic mucosal FHC cells. Representative data is shown. (C) PRMT5 protein expression level is quantified. **P < 0.01 vs. FHC cells. (D) qRT-PCR analysis of PRMT5 mRNA expression level in human colorectal cancer tissues and adjacent normal tissues (n=25-30). **P < 0.01 vs. normal tissues. (E) PRMT5 protein expression level is analyzed by Western blotting in human colorectal cancer tissues and adjacent normal tissues (n=7). Representative data is shown. (F) PRMT5 protein expression level is quantified. **P < 0.01 vs. normal tissues. (G) The global symmetric dimethylarginine (SDMA) is detected by Western blotting. Representative data is shown.