Research Paper Volume 13, Issue 3 pp 4482—4502

Figure 5. Analysis of the ACK1 signaling pathways. RNA-seq data of 188 lung cancer cell lines were retrieved from the CCLE database. GSEA was performed after dividing cell lines into ACK1^high and ACK1^low groups by the average ACK1 expression level (A). Principal component analysis (PCA) of the 57 hub genes (degree≥20) was carried out in the TCGA-LUAD (B) and TCGA-LUSC (C) versus normal tissues. The most significant 14-gene module was derived from DEGs, which captured 50 coexpressed genes in the TCGA-LUAD cohort (D). Most of the 64 genes were enriched in the ubiquitin-mediated proteolysis and the proteasome (E), which was validated by GSEA using CCLE lung cancer cell data (F).