Research Paper Volume 13, Issue 3 pp 3239—3253

Figure 3. Flow cytometry analysis of MMU and NMR cells upon treatment with DNA damaging agents. (A). NMR and mouse cells were stained with FITC-Annexin V (AnnV) and Propidium Iodide (PI). The gating strategy for double negative AnnexinV^-/PI^-(Q3), single positive, early apoptotic cells (AnnexinV⁺/PI^-) (Q4), single positive (AnnexinV^-/PI⁺) (Q1) and double positive AnnexinV⁺/PI⁺ (Q2) is shown. (B–G). Cell death of MMU (B–D) and NMR (E–G) cells under the wide spectrum of cytotoxic conditions was evaluated by FACS analysis. The total number of cells was taken as 100%; blue color indicates the population of viable cells, double negative AnnexinV^-/PI^-(Q3); yellow color is used for single positive, early apoptotic cells (AnnexinV⁺/PI^-) cells (Q4); red color is implemented for double positive AnnexinV⁺/PI⁺ cells (Q2); purple color for single positive (AnnexinV^-/PI⁺) cells (Q1). The representative experiment out of three independent ones is shown.