Research Paper Volume 13, Issue 9 pp 12766—12779

Figure 6. Effect of DDX49 on HCC cell lines treated with morphine. QGY-7703 cells were transfected with LV-DDX49-KD (KD) or LV-DDX49-OE (OE) or the corresponding negative control lentivirus (NC-KD, NC-OE), then treated with morphine (10μM) for 5 days. (A) Proliferation was assessed using the MTT assay. OD490, optical density at 490 nm. (B) Apoptosis rates in the different groups were assessed using flow cytometry. (C) Cell cycle in the different groups were assessed using flow cytometry. *P < 0.01 compared to the respective control group. CON cells were derived cells without any treating. Morphine QGY-7703 cells were treated with morphine (10μM) for 48h. NC-KD+M cells were knock down using negative control vector and treat with morphine for 48h. KD+M cells were knock down of DDX49 using lentivirus and treat with morphine for 48h; OE+M cells were transfected with over-expression of DDX49 lentivirus vector and treat with morphine with 48h; NC-OE+M cells were transfected over-expression of DDX49 negative control vector and treat with morphine for 48h.