Research Paper Volume 13, Issue 11 pp 14924—14939

Figure 5. MiR-146a-3p suppresses endometrial receptivity. (A) CCK-8 was used to detect the effect of miR-146a-3p on the proliferation of EEC cells. (B) The effect of miR-146a-3p on the proliferation of EEC cells was detected by the clone formation assay. (C) The effect of miR-146a-3p on the apoptosis of EEC cells was detected by flow cytometry. (D) The scratch assay was used to detect the effect of miR-146a-3p on the invasion of EEC cells. (E) Transwell assays were used to detect the effect of miR-146a-3p on the invasion of EEC cells. (F) The effect of miR-146a-3p overexpression on the expression of receptivity-related factors in EEC cells was detected by real-time quantitative PCR. (G) Western blotting was used to detect the effect of miR-146a-3p on the expression of receptivity-related factors in EEC cells. (H) Real-time quantitative PCR was used to detect the effect of miR-146a-3p knockdown on the expression of receptivity-related factors in EEC cells. (I) Western blotting was used to detect the effect of miR-146a-3p knockdown on the expression of receptivity-related factors in EEC cells. (J) The effect of knockdown of miR-146a-3p on LIF expression in EEC cell culture medium was detected by ELISA. (K) Effect of miR-146a-3p overexpression on the trophoblast adhesion rate. (L) Effect of miR-146a-3p inhibition on the trophoblast adhesion rate. (M) Effect of miR-146a-3p on embryo implantation. "^*" indicates P < 0.05, "^**" indicates P < 0.01, and "^***" indicates P < 0.001.