Research Paper Volume 13, Issue 11 pp 15100—15113

Figure 2. Knockdown of hsa_circ_0032131 reversed the anti-proliferative effect of IL-1β on CHON-001 cells. (A) CHON-001 cells were transfected with hsa_circ_0032131 shRNA1 or hsa_circ_0032131 shRNA2 for 48 h. Afterward, the expression of hsa_circ_0032131 in CHON-001 cells was detected by RT-qPCR. (B) CHON-001 cells were treated with IL-1β, hsa_circ_0032131 shRNA1, or IL-1β + hsa_circ_0032131 shRNA1. Next, the expression of hsa_circ_0032131 in CHON-001 cells was detected by RT-qPCR. (C) Cell viability was tested by CCK-8 assay. (D) The proliferation of CHON-001 cells was measured by Ki67 staining (red fluorescence). Blue fluorescence indicates DAPI. (E) The number of Ki67-positive cells was calculated. ^**p < 0.01 compared with the control. ^##p < 0.01 compared with IL-1β.