Research Paper Volume 13, Issue 11 pp 15100—15113

Figure 3. IL-1β-induced CHON-001 growth inhibition was reversed by hsa_circ_0032131 knockdown. (A, B) Apoptosis of CHON-001 cells was measured by flow cytometry. (C) The expression of Aggrecan, MMP-13, Bax, and active caspase 3 in CHON-001 cells was detected by western blotting. (D) The relative expression of MMP-13 was quantified by normalizing it to that of β-actin. (E) The relative expression of Bax was quantified by normalizing it to that of β-actin. (F) The relative expression of active caspase 3 was quantified by normalizing it to that of β-actin. (G) The relative expression of Aggrecan was quantified by normalizing it to that of β-actin. ^**p < 0.01 compared with the control. ^##p < 0.01 compared with IL-1β.