Research Paper Volume 13, Issue 11 pp 15100—15113

Figure 4. Hsa_circ_0032131 sponged miR-502-5p in CHON-001 cells. (A) Gene structure of hsa_circ_0032131 at position 69 to 75 indicated the predicted target site of miR-502-5p in its 3'UTR. (B) CHON-001 cells were transfected with miR-502-5p agomir/antagomir. Next, the expression of miR-502-5p in CHON-001 cells was measured by RT-qPCR. (C) The luciferase activity was measured in CHON-001 cells using the dual-luciferase reporter assay following co-transfection with WT/MT hsa_circ_0032131 3′-UTR plasmid and miR-502-5p. (D) The co-localization of hsa_circ_0032131 and miR-502-5p was detected by FISH. ^**p < 0.01 compared with the control.