Research Paper Volume 13, Issue 11 pp 15479—15490

Figure 1. SIRT1 expression is decreased in macrophages following allergen exposure. (A) Schematic map of allergic asthma model; (B) Representative IHC images of SIRT1 in lung tissue of mice (scale bar, 100 μm); (C) Semiquantification of IHC was done using Image Pro 6.1 software; (D, E) SIRT1 expression in lung tissues of Sirt1^fl/fl mice were assessed using Western blot and RT-PCR analysis; Representative immunofluorescence images of SIRT1 expression in alveolar macrophages are revealed using F4/80 in lung tissues (F) and BALF (H) of mice (Scale bar, 100 μm and 20 μm, respectively); (G, I) Semiquantification of immunofluorescence images was done using Image Pro 6.1 software. Data are presented as Mean ± SEM of three independent experiments (n = 5-8 for each group). **P<0.01 and ***P<0.001.