Research Paper Volume 13, Issue 11 pp 15501—15510

Figure 6. BCRT1 induced osteosarcoma cell cycle and proliferation via modulating FGF7 expression. (A) The level of FGF7 in four osteosarcoma cell lines (MG-63, HOS, SAOS-2 and U2OS) and hFOB was detected using qRT-PCR assay. (B) The expression of FGF7 was determined by qRT-PCR assay. (C) The expression of cyclin D1 was measured using qRT-PCR assay. (D) The expression of CKD2 was measured using qRT-PCR assay. (E) The level of ki-67 was detected using qRT-PCR assay. (F) Cell proliferation was measured by CCK-8 assay. (G) Inhibition expression of FGF7 suppressed cell cycle in BCRT1-overexpressing MG-63 cell. *p<0.05, **p<0.01 and ***p<0.001.