Research Paper Volume 13, Issue 11 pp 15501—15510

Figure 8. FGF7 induces osteosarcoma cell cycle and proliferation, EMT progression and secretion of inflammatory mediators. (A) The expression of FGF7 was measured by qRT-PCR assay. (B) Ectopic expression of FGF7 increased cell growth in the MG-63 cell. (C) The expression of ki-67 was measured by qRT-PCR assay. (D) Overexpression of FGF7 increased cell cycle in MG-63 cell. (E) Elevated expression of FGF7 suppressed E-cadherin expression and increased the expression of N-cadherin and vimentin in MG-63 cell. (F) The level of IL-1β was determined by ELISA assay. (G) The level of IL-6 was determined by ELISA assay. (H) The level of TGFβ was determined by ELISA assay. *p<0.05, **p<0.01 and ***p<0.001.