Research Paper Volume 13, Issue 11 pp 15511—15522

Figure 3. Propofol promotes M2 polarization of macrophages in vitro. PMs incubated with Pro or vehicle were kept and under normoxia or exposed to 6-h hypoxia followed by 24-h reoxygenation (H/R). (A–C) ELISA measurements of TNF-α, IL-6, and IL-10 levels in culture supernatants. (D–F) Real-time PCR assessment of iNOS, Arg1, and Mrc1 mRNA expression in cell lysates. (G, H) Representative western blot images and corresponding densitometric analysis of total and phosphorylated STAT 1/3/6 and PPARγ expression in cell lysates. Data were compared to control (Con) and GAPDH was used as loading control. Con: Normoxia+PBS; Pro: Normoxia+Pro; H/R: H/R+PBS. *P < 0.05 between the indicated groups.