Research Paper Volume 13, Issue 11 pp 14590—14603

Figure 1. Representative images and visualisations showing the cell detection of proliferating CD8⁺ T-lymphocytes. Multiplex immunofluorescence images (A, B) showing CD8⁺ cytotoxic T-lymphocytes (white) and Ki67⁺ proliferating cells (red) with a low (A, C) and a high (B, D) percentage of proliferating Ki67⁺CD8⁺ T-cells. The visualization (C, D) of the digital image analysis underlines the accuracy of the automated detection of the subset of Ki67⁺CD8⁺ proliferating cytotoxic T-cells (blue). 400x magnifications are shown in the insets.