Research Paper Volume 13, Issue 13 pp 17428—17441

Figure 4. CircRNA FUT10 suppresses SkMSC proliferation and differentiation by targeting miR-365a-3p. (A) circRNA FUT10 overexpression vectors, miR-365a-3p mimics, and circRNA FUT10 + miR-365a-3p were transfected into SkMSCs. miR-365a-3p expression was examined by real-time qPCR. (B) EdU assay results indicated that upregulated circRNA FUT10 suppressed SkMSC proliferation by regulating miR-365a-3p. (C) Quantification of EdU-positive cells. (D) MyHC and MyoD mRNA were examined by real-time qPCR. (E) Western blot analysis showing MyHC and MyoD protein expression in different groups. (F) Quantification of MyHC and MyoD were normalized to β-actin. Each bar represents the mean ± SEM. *P < 0.05, **P < 0.01. All experiments were performed at least three times with duplication within each individual experiment.