Research Paper Volume 13, Issue 13 pp 17428—17441

Figure 5. HOXA9 is a direct miR-365a-3p target. (A) Potential miR-365a-3p targets were identified in the HOXA9 3′ UTR using TargetScan software. (B) Adult and aged (C) SkMSCs were transfected with both miR-365a-3p mimic and wild-type or mutant HOXA9 3′ UTR. Binding activity was detected by dual-luciferase activity. (D) RNA pull-down assay results indicated that HOXA9 binding was enriched on miR-365a-3p probes. (E) miR-365a-3p mRNA was examined by real-time qPCR. (F) qPCR and (G) western blotting were performed to evaluate HOXA9 mRNA and protein expression in transfected cells. (H) Quantification of HOXA9 was normalized to β-actin. NC-antagomir, NC-mimics: control group. *P < 0.05, **P < 0.01. All experiments were performed at least three times with duplication.