Research Paper Volume 13, Issue 13 pp 17428—17441
CircRNA FUT10 regulates the regenerative potential of aged skeletal muscle stem cells by targeting HOXA9
Figure 8. CircRNA FUT10 regulated HOXA9 by sponging miR-365a-3p. (A) SkMSCs were transfected with negative control (NC) miRNA, circRNA FUT10vector, miR-365a-3P mimic, (circ-OE), and circ-OE+miR-365a-3p. HOXA9 mRNA was examined by qPCR. (B) Western blot analysis showing HOXA9 protein expression in different groups. (C) Quantification of HOXA9 were normalized to β-actin. (D) SkMSC proliferation was measured by EdU assays. (E) Quantification of EdU-positive cells. (F) MyHC and MyoD mRNA were examined by real-time qPCR. (G) Western blot analysis showing MyHC and MyoD protein expression in different groups. (H) Quantification of MyHC and MyoD were normalized to β-actin. *P < 0.05, **P < 0.01. Each experiment was performed at least three times.