Research Paper Volume 13, Issue 13 pp 17462—17472

Figure 4. miR-30a-5p suppresses ATG5-mediated autophagy in LUSC. (A) The result of mRNA were used to evaluate the effect of miR-30a-5p on ATG5. (B) SW900 and NH91 cells were transfected with mimic NC or mimic miR-30a-5p and then starved for 2 hours to stimulate autophagy. ATG5, P62, Beclin 1, and LC3B I/II expression were evaluated by western blot. (C) GFP-mRFP-LC3 lentivirus was used to evaluate autophagy flux in mimic NC/miR transfected SW900 cells. Red or yellow puncta indicating complete/incomplete autophagy were calculated and reported as the mean ± SD. All the P values were compared with the control ^*P < 0.05, ^**P < 0.01, ^***P < 0.0001.