Research Paper Volume 13, Issue 13 pp 17830—17846
Linc00941 regulates esophageal squamous cell carcinoma via functioning as a competing endogenous RNA for miR-877-3p to modulate PMEPA1 expression
Figure 3. Linc00941 targeted miR-877-3p and repressed the expression of miR-877-3p. (A) The common miRNAs that could be targeted by linc00941 as predicted by the lncRNASNP2 and LncBase V.2 online predicting tools. (B) The predicted binding sites between linc00941 and miR-877-3p. The red letters showed the mutant sites in linc00941 segment used for constructing the mutant luciferase reporter vector. (C, D) Luciferase activity of linc00941-wt reporter vectors and linc00941-mut reporter vectors in KYSE-510 cells after transfecting with different miRNAs. (E) Knockdown of linc00941 up-regulated miR-877-3p expression in KYSE-510 cells as determined by qRT-PCR. (F) MS2-RIP assay followed by qRT-PCR to determine miR-877-3p that endogenously associated with linc00941 in KYSE-510 cells. N = 3. **P<0.01 and ***P<0.001 compared between different treatment groups.