Figure 2. Expression levels of RUFY3 in HCC cell and the effect of RUFY3 expression on NF-κB signaling-related markers. (A) qRT-PCR detection of RUFY3 expression in five HCC cell lines and one normal liver cell line. (B) Western blot analysis of RUFY3 expression in five HCC cell lines and one normal liver cell line. (C) Western blot analysis of the effect of RUFY3 down-regulation or up-regulation on NF-κB signaling-related markers. *P<0.05.