Figure 4. Apoptosis analysis of the mutation p.Pro326Leu. RGC-5 cells were transfected with wild-type PCK2 or p.Pro326Leu mutation, respectively. Multiple apoptotic bodies (a, pink dots) were observed in the cells transferred with p.Pro326Leu mutation (A), and the apoptosis was further proved by flow cytometry (B). *P < 0.05.