Extracorporeal shockwave relieves endothelial injury and dysfunction in steroid-induced osteonecrosis of the femoral head via miR-135b targeting FOXO1: <i>in vitro</i> and <i>in vivo</i> studies

Xinjie Wu; Yanlei Wang; Xiaoyu Fan; Xin Xu; Wei Sun

doi:doi:10.18632/aging.203816

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Research Paper Volume 14, Issue 1 pp 410—429

Extracorporeal shockwave relieves endothelial injury and dysfunction in steroid-induced osteonecrosis of the femoral head via miR-135b targeting FOXO1: in vitro and in vivo studies

Figure 6. Role of miR-135b in the regulation of FOXO1 and related proteins. After transfection of inhibitor mir-135b, ECs were subjected to ESWT with 0.05 mJ/mm², 1000 shots followed by DEX with 180 μM. (A–J) Immunoblotting and statistical graph of FOXO1, Cyclin D1, Bim, Bcl2, Bax, cleaved Caspase-3, MMP2, MMP9 (N-terminal), Cyclin E1, and VEGFA. n=3 ^**P < .01, ^***P < .001.