Research Paper Volume 14, Issue 4 pp 1767—1781
The novel lncRNA GPC5-AS1 stabilizes GPC5 mRNA by competitively binding with miR-93/106a to suppress gastric cancer cell proliferation
Figure 1. GPC5-AS1 is down-regulated in GC and overexpression of GPC5-AS1 inhibits cell proliferation in vitro. (A, B) The expression of GPC5-AS1 in GC tissue samples and cell lines was measured by qRT-PCR. (C) Measurement of GPC5-AS1 expression level in SGC-7901 cells with pc-GPC5-AS1. (D) Measurement of GPC5-AS1 expression level in BGC-823 cells with indicated shRNA. (E) Colony formation, (F) MTT assay, (G) cell apoptosis, and (H) cell cycle assay were performed in SGC-7901 cells with pc-GPC5-AS1. (I–L) Cell function analyses were determined in BGC-823 cells with sh-GPC5-AS1. (p* < 0.05, p** < 0.01).